REGENERATIVE MEDICINE METHODOLOGY

Course Code:

8101

Semester:

8th Semester

Specialization Category:

SC

Course Hours:

3

ECTS:

6


Course Tutors

Venetikou Maria

LEARNING OUTCOMES

After the end of the course, the student will be able to:
• know the use of primitive hematopoietic cells in the treatment of various diseases and what are the prospects for new treatments.
• know how to obtain the primordial hematopoietic cells.
• know how to use primitive hematopoietic cells in autologous and heterologous transfusion.
• know the Greek, European and international services that deal with banks of primitive hematopoietic cells and their cooperation.
• know the analyses that are done to control the quality of the genetic material before cryopreservation.
• know the procedures of cryopreservation of biological material.
• know the legal conditions for the operation of cryopreservation banks of primitive hematopoietic cells and what accreditations and certifications they can have.

SYLLABUS

Theory
1. The primordial hematopoietic cells (stemcells). What are hematopoietic stem cells? How and where they are produced, what are their biological properties and how they differ from each other.
2. Transfusion of primitive hematopoietic cells. Autologous and heterologous transfusion of hematopoietic cells. Which is chosen in each case and how are they made.
3. The therapeutic value of primitive hematopoietic cells (I). What diseases are proven to be cured by them.
4. The therapeutic value of primitive hematopoietic cells (II). What research is currently being done on the therapeutic use of primitive hematopoietic cells in diseases where they have not yet been exploited?
5. The reception of primitive hematopoietic cells. How, where and when the primordial hematopoietic cells can be obtained from doctors, health professionals and from the donors themselves.
6. The banks of primitive hematopoietic cells, tissues and blood (stem cells). How are they organized in Greece and with which legal framework?
7. Differences between stem cell, tissue and blood banks. What are the differences between them, in collection, cryopreservation methods, etc.
8. The global Netcord network and the European Eurocord network. What is the global collaboration to search for stem blood cells to give to patients who need them.
9. The isolation of primordial hematopoietic cells from the original sample. What tests are done after receiving the primordial hematopoietic cells.
10. Cryopreservation. The process of freezing and thawing primordial hematopoietic cells in liquid nitrogen refrigerators.
11. Private “stem cell” banks. To whom they are addressed, what they can offer. The individual and family storage of “stem cells”. What is the role of the Association of Hellenic Umbilical and Placental Blood Banks (EETOA).
12. What certifications and accreditations can hematopoietic stem cell banks have? Which of them are mandatory for their operation?
13. The accreditation-certification of banks of primitive hematopoietic cells. What are the basic technical requirements?