GENERAL ANALYSIS OF BIOLOGICAL SAMPLES

Course Code:

4051-4052

Semester:

4th Semester

Specialization Category:

SC

Course Hours:

4

ECTS:

4


LEARNING OUTCOMES

The student at the end of the lesson will be familiar with the:

  • general urine analysis,
  • general feces analysis,
  • general sperm analysis,
  • microscope analysis of vaginal fluid, pericardial fluid, pleuritical fluid, perinate fluid, cerebrospinal fluid and articular fluid.

 

SYLLABUS

Τheoretical courses
1. Introduction in analysis of urine, uroscopy.
2. Introduction in general analysis of urine. The collection of urine samples. The physical properties of urine. The centrifugation of urine samples.
3. Microscopy of urine. The identification of cell and non-organic substances of urine’s sentiment.
4. Diagnosis of basic diseases of urinary system. The diagnosis of mellitus, icterus, urine stones.
5. Kidney diseases, their diagnosis though general analysis of urine.
6. Automatic analysis of urine. Internal and external quality control of urine analysis.
7. General analysis of vaginal samples (the microscopic elements of wet and dyed smears).
8. General analysis of amniotic fluid. The microscopic and chemical investigation of amniotic fluid.
9. Chemical analysis of maternal milk. The chemical analysis of chlorides in sweet.
10. Cytological and microscopical analysis of exudates.
11. The general analysis of feces and sputum.
12. Τhe general analysis of cerebrospinal fluid.
13. The general analysis of synovial fluid.

 

Laboratory lessons
1. The use of urine strips, the collection of urine samples. Laboratory practice in the use of microscope.
2. The physical properties of urine (color, sediment, turbidity), determination of urine glucose with Benedict reagent. Laboratory practice in general urine analysis.
3. Centrifugation and microscopy of urine sediment. Laboratory practice in general urine analysis.
4. Τhe chemical determination of protein and hemoglobulin in urine samples. Laboratory practice in general urine analysis.
5. The chemical determination of ketones and bilirubin in urine samples. Laboratory practice in general urine analysis.
6. The general analysis of vaginal fluid.
7. Τhe general analysis of synovial fluid.
8. The general analysis of cerebrospinal fluid.
9. The microscopic and cytological analysis of pleural fluid.
10. The microscopic and cytological analysis of pericardial fluid.
11. The microscopic and cytological analysis of pancreatic fluid.
12. The chemical analysis of gastric fluid.
13. The microscopic and chemical analysis of feces.