PRINCIPLES OF INSTRUMENTAL ANALYSIS

Course Code:

3077-3078

Semester:

3rd Semester

Specialization Category:

SBC

Course Hours:

6

ECTS:

7


LEARNING OUTCOMES

The student, with the successful completion of the course, should:

  • know the types of instrumental methods of analysis.
  • have acquired knowledge of the powerful and elaborate collection of tools, for qualitative and quantitative analysis of the matter.
  • have understood the basic operation principles of measuring instruments.
  • be able to compare the capabilities, the advantages and disadvantages of various analysis techniques.
  • be able to understand the possible sources of errors, that follow the measurements.
  • Understand the limitations of sensitivity, repeatability and accuracy of instrumental measurements.

 

SYLLABUS

THEORETICAL PART

Introduction
Classification of Analytical Methods-Types of Instrumental Methods of Analysis-Analytical Instruments- Selection of an Analytical Method- Calibration in Instrumental Methods- Signal and Noise

Atomic Spectroscopy I
Introduction to Spectroscopic Techniques- Organology- Atomic Absorption and Atomic Fluorescence Spectrometry- Atomic Emission Spectrometry

Atomic Spectroscopy II
Atomic Mass Spectrometry- X-ray Spectrometry

Molecular Spectroscopy I
Introduction to Ultraviolet / Visible Molecular Absorption Spectrometry (UV / Vis)- Applications of Ultraviolet / Visible Molecular Absorption Spectroscopy (UV / Vis)- Molecular Luminesence Spectrometry

Molecular Spectroscopy II
Introduction to Infrared (IR) Spectrometry- Applications of Infrared Spectrometry- Raman Spectroscopy

Molecular Spectroscopy III
Nuclear Magnetic Resonance Spectroscopy- Molecular Mass Spectrometry- Electron Microscopy

Separation Methods I
Introduction to Chromatographic Separations- Gas Chromatography

Separation Methods II
High Performance Liquid Chromatography (HPLC)

Separation Methods III
Extraction and Supercritical Fluid Chromatography- Electrophoresis and Capillary Electrochromatography

Electroanalytical Chemistry I
Introduction to Electroanalytical Chemistry- Coulometry

Electroanalytical Chemistry II
Voltametry- Potentiometry

Radiochemical and Thermal Methods
Automated Analysis Methods

 

LABORATORY PART

Ultraviolet / Visible Molecular Absorption Spectrometry: qualitative analysis, obtaining a spectrum of substance.
Ultraviolet / Visible Molecular Absorption Spectrometry: Quantitative analysis of proteins.
Atomic Emission Spectrometry: Quantitative analysis of Na+ and K+ quantification by flame photometry
Infrared (IR) Spectrometry: Analysis of uroliths.
High Performance Liquid Chromatography (HPLC), Reverse Phase: Quantitative determination of caffeine.
Automatic analyzer: Determination of enzymes, lipids, glucose and electrolytes.